Mechanism Of Interaction Between Pioglitazone Hydrochloride And Bovine Transferrin By Spectroscopic And Molecular Docking Methods

Authors

  • C.D. Wang
  • B. Liu
  • G. Bian
  • L.H. Ma
  • C. Zhang
  • X. Cheng D

Keywords:

Spectrometry, Molecular Docking, Pioglitazone Hydrochloride, Bovine Transferrin, Interaction Mechanism

Abstract


The binding of pioglitazone hydrochloride with bovine transferrin was investigated by spectroscopic and molecular docking methods under different temperature conditions (298, 310 and 318 K). The results demonstrate that the interaction between pioglitazone hydrochloride and bovine transferrin is taking place via static quenching with 1:1 binding ratio. The fluorescence data were treated by using the double logarithmic equation, and the binding constants Ka of the interaction of pioglitazone hydrochloride-bovine transferrin systems and the number of binding sites n were obtained. The thermodynamic parameters of pioglitazone hydrochloride-bovine transferrin systems under different temperatures were obtained by the thermodynamic equation. The experimental data show that the interactions between them are mainly electrostatic force interaction, which is consistent with the molecular docking results.

Downloads

Published

2018-12-19

How to Cite

Wang, C., Liu, B., Bian, G., Ma, L., Zhang, C., & Cheng D, X. (2018). Mechanism Of Interaction Between Pioglitazone Hydrochloride And Bovine Transferrin By Spectroscopic And Molecular Docking Methods. Journal of Engineering and Technology (JET), 9(2), 61–76. Retrieved from https://jet.utem.edu.my/jet/article/view/3694

Issue

Vol. 9 No. 2 (2018): JOURNAL OF ENGINEERING AND TECHNOLOGY

Section

Articles

License

Thank you for your interest in submitting your manuscript to the Journal of Engineering and Technology (JET).

JET publishes only original works. Manuscripts must not be previously published or under consideration by any other publications. Papers published in JET may not be published again in whole or in part without permission. Please review these guidelines for researching, writing, formatting and submitting your manuscript. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0).

Those submitting manuscripts should be carefully checked to ensure that all works contributed to the manuscript are acknowledged. The list of authors should include all those who can legitimately claim authorship. Manuscript should only be submitted for consideration once consent is given by all contributing authors using Transfer of Copyright form.