Polystyrene Particles and Mammalian Cells Motion Behaviour on Different Surfaces
The stochastic motion behaviour of polystyrene particles and teratocarcinoma cells on plain and functionalised surfaces is investigated and analysed. The solution of 1 x 106 particles/cells per ml concentration is pipetted into a reservoir and images are captured and analysed using an in-house written software. A theoretical model was used to predict the motion and compared to the experimental results. The conditions and limitations to allow particles and cells to move freely in stochastic motion on surface are discussed in this paper. PEG functionalisation of the glass surface was found to improve the particles and cells mobility, on average 26%. Analysis technique proposed in this paper demonstrates that size distribution of different cell line can be determined. The results are presented in light of the potential application of the observed motion on functionalised surfaces for lab-on-a-chip devices, especially for adherent biological cells applications.
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